Journal: Cell metabolism

Article Title: Fibroblasts mobilize tumor cell glycogen to promote proliferation and metastasis

doi: 10.1016/j.cmet.2018.08.007

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: pLKO.1 TRC control , Addgene , Cat#10879.

Techniques: Virus, Recombinant, Mutagenesis, Colorimetric Assay, Bioassay, Phospho-proteomics, Plasmid Preparation, Control, Software

Journal: Scientific Reports

Article Title: Lineage tracing analysis of cone photoreceptor associated cis-regulatory elements in the developing chicken retina

doi: 10.1038/s41598-019-45750-7

Figure Lengend Snippet: Quantification of lineage trace recombination efficiencies mediated by FlpE, Cre and PhiC31. ( a ) Schematic representation of recombinase-based lineage tracing strategy. Red triangles represent recombinase target sequences. ( b – d ) Quantification of FACS-analyzed dissociated retinal cells electroporated with a UbiqC::TdTomato electroporation control, a FlpE, Cre, or PhiC31 recombinase plasmid, and the corresponding responder vector. Retinas were electroporated at E5 and fixed after two days in culture. ( b ) Quantification of basal recombination, with recombinase plasmids driven by a basal promoter (TATA box). Error bars represent 95% confidence intervals, n = 12, p < 0.001 upon Kruskal Wallis test with post hoc Dunn test. ( c ) Quantification of enhancer-driven recombination, with recombinase plasmids driven by the ThrbCRM1 enhancer element. Error bars represent 95% confidence intervals, n = 6. ( d ) Quantification of ubiquitous recombination, with recombinase plasmids driven by the ubiquitous CAG promoter. Error bars represent 95% confidence intervals, n = 6. CRM, cis-regulatory module; bp, basal promoter; pA, polyadenylation sequence.

Article Snippet: Bp::PhiC31 was made by PCR-amplifying the PhiC31 coding region from pPhiC31o (Addgene plasmid #13794 was a gift from Philippe Soriano) using an Xma1-tagged forward primer and a BsrG1-tagged reverse primer (see Supplementary Table for all primer sequences).

Techniques: Electroporation, Control, Plasmid Preparation, Sequencing

Journal: Scientific Reports

Article Title: Lineage tracing analysis of cone photoreceptor associated cis-regulatory elements in the developing chicken retina

doi: 10.1038/s41598-019-45750-7

Figure Lengend Snippet: In ovo lineage trace of 3 Thrb CRMs yields unique patterns of activity. ( a – e ) Representative images of vertically sectioned retinas that were electroporated in ovo at E3 with CAG::nucβgal as an electroporation control, bp::PhiC31 ( a ), CAG::PhiC31 ( b ), ThrbCRM1::PhiC31 ( c ), ThrbCRM2::PhiC31 ( d ), or ThrbICR::PhiC31 ( e ) and CAaNa::GFP. Embryos were grown until E10, and all images are maximum intensity projections. To the right of each image is a schematic of the chick retina with recombined cell types from all counted images colored in green. ( f ) Quantification of the % recombination in each retinal layer, for each of the conditions assessed, n = 3–8. ( g ) Quantification of the % of representation for each retinal cell type among the recombined cells for that particular condition (GFP+ cells of one cell type/all GFP+ cells *100). The negative control had no recombination (f) so it was excluded here. Error bars represent SEM, n = 3–8. ONL, outer nuclear layer; INL, inner nuclear layer; GCL, ganglion cell layer; bp, basal promoter; PRs, photoreceptors; HCs, horizontal cells; ACs, amacrine cells; BCs, bipolar cells; RGCs, retinal ganglion cells.

Article Snippet: Bp::PhiC31 was made by PCR-amplifying the PhiC31 coding region from pPhiC31o (Addgene plasmid #13794 was a gift from Philippe Soriano) using an Xma1-tagged forward primer and a BsrG1-tagged reverse primer (see Supplementary Table for all primer sequences).

Techniques: In Ovo, Activity Assay, Electroporation, Control, Negative Control